Celine Dion quashes rumours about pregnancy, though she would like to extend her family.
Rene Angelil confirmed to Canadian newspaper Journal de Montreal that the latest in vitro fertilization treatment she underwent in October was unsuccessful.
"We're living the reality of the majority of couples who face these procreation techniques," he told the paper.
She and Angelil have an 8-year-old son, Rene-Charles, who was conceived by in vitro fertilization after six years of trying. While trying to conceive her first child, she had her embryos frozen in liquid nitrogen.
When she finished her concert run in Las Vegas in 2007, she consulted Dr. Zev Rosenwaks in New York City about trying to conceive again.
The 41-year-old singer singer (who is the youngest of 14 children) has long talked about wanting to have more kids.
Us Magazine has contributed to the report.