Huntelaar was hurt during a morning training session and immediately left the field to ice his leg. He did not return to training, the Royal Dutch Soccer Association said on its Web site. The extent of the injury was not immediately known.
First-choice striker Ruud van Nistelrooy and backup forward Danny Koevermans trained separately from the team because of slight injuries, but are expected to be available for Saturday's match. Coach Marco van Basten is also missing the injured Arjen Robben and Robin van Persie.
Four other players Wesley Sneijder of Real Madrid, Demy de Zeeuw of AZ Alkmaar, Wilfred Bouma of Aston Villa and Kew Jaliens of AZ have recovered from minor knocks and went through full training. They are expected to be fit for Saturday's match in Rotterdam and the team's final qualifier against Belarus in Minsk next Wednesday.
Van Basten's team is second in Group G behind Romania and needs just two points from its last two matches to qualify for next year's tournament.
The co-author of this disaster is the Dutch government, which did not find either strength or desire to save the lives of its citizens who were flying on that plane. The Dutch authorities did not demand Ukraine to comply with international aviation regulations
On the second day of the St. Petersburg International Economic Forum, a plenary meeting was held, in which Russian President Vladimir Putin, French President Emmanuel Macron, Japanese Prime Minister Shinzo Abe, Chinese Vice President Wang Qishan and IMF head Christine Lagarde took part